产品名称

Mitochondrial 线粒体 DNA分离试剂盒

样品类型

Tissue, Adherent cells, Suspension cells

检测类型

Direct

检测时间

3h 00m

产品概述

Mitochondrial DNA Isolation Kit ab65321 provides convenient tools for isolating mtDNA from a variety of cells and tissues in high yield and purity, without contaminations from genomic DNA. The purified mtDNA can be used for a variety of studies such as enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications

Please note: One kit is sufficient for 50 samples if Adherent cell or Suspension cells are used and for 25 samples if tissue is used.

Mitochondrial DNA isolation protocol summary:

- collect cells by centrifugation at 600 x g for 5 min and wash with PBS

- centrifuge for 5 min and resuspend pellet in cytosol extraction buffer

- incubate on ice for 10 min

- homogenize in dounce tissue grinder

- centrifuge for 10 min at 700 x g to pellet nuclei and intact cells

- transfer supernatant and centrifuge at 10,000 g for 30 min

- remove supernatant, resuspend pellet in cytosol extraction buffer and centrifuge at 10,000 g for 30 min

- resuspend pellet (mitochondria) and lyse in mitochondrial lysis buffer for 10 min

- add enzyme mix and incubate for 60 min

- add ethanol and incubate for 10 min

- centrifuge for 5 min and retain pellet (mitochondrial DNA)