Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
Use 10x Tris/Glycine Buffer as a transfer buffer for western blots or as a running buffer for native protein gel electrophoresis.
For tank or semi-dry blotting for SDS PAGE gels, usually with the addition of 20% methanol
For tank blotting of native gels, without methanol
As a running buffer for native gels